Pimelea simplex

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Scientific/model-organism use:
Pimelea simplex has been employed as a source of genetic material in molecular phylogenetic investigations of the family Thymelaeaceae. DNA is routinely extracted from fresh or silica‑dried leaf tissue, and sequence data for a suite of standard plant markers—chloroplast rbcL (≈1,428 bp open reading frame), matK (≈1,500 bp), and the nuclear ribosomal internal transcribed spacer (ITS, ≈650 bp)—have been deposited in public repositories such as GenBank and the National Center for Biotechnology Information (NCBI). These sequences are incorporated into family‑level phylogenies that address intra‑generic relationships within Pimelea, the diversification of Australian Thymelaeaceae, and the placement of the genus relative to other Malvales lineages. Multi‑locus datasets from P. simplex have been used to test hypotheses concerning historical biogeography, species delimitation, and the evolution of morphological traits in the family. In several systematic studies the species has also served as an outgroup taxon for rooting trees of related genera (e.g., Dais, Edgeworthia, Wikstroemia), providing a basal reference point that improves resolution of deep nodes within the order. The available DNA sequences are referenced in peer‑reviewed articles that contribute to the Angiosperm Phylogeny Group (APG) framework and are available for comparative analyses of genome evolution, including investigations of chloroplast gene order, intron gain/loss events, and substitution rates.

Marker choice: The rbcL region is highly conserved across angiosperms and provides a backbone for deep relationships, whereas matK and the ITS region offer higher resolution for intra‑generic divergence. Combined datasets (rbcL+matK+ITS) have become a standard for Thymelaeaceae phylogenetics, and the inclusion of P. simplex improves taxon sampling and branch support. Specimens used for DNA extraction are typically archived in Australian herbaria (e.g., NSW, MEL), with voucher numbers recorded in GenBank entries, ensuring traceability and reproducibility of data. The accumulated sequences are part of community resources such as the Global Biodiversity Information Facility (GBIF) and the Atlas of Living Australia, facilitating comparative analyses across the family and beyond. Because the plant is a non‑cultivated wild shrub, no cultivated, commercial, or industrial product is recorded for P. simplex, and its documented use is confined to basic research in plant systematics and evolutionary biology.

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No reference genome is available on NCBI yet. We are constantly monitoring for new data.

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